There are often times when there is not enough hours in a day to initiate and complete all of those critical experiments which are an integral part of your research program. Dr. J. Thomas McClintock, the founder of DNA Diagnostics, Inc., offers laboratory support services, technical advice, and scientific consultation in a timely and economic manner through DNA Diagnostics, Inc. In partnership with other DNA typing laboratories, Dr. McClintock of DNA Diagnostics, Inc. is dedicated to obtaining quality DNA test results based on sound science and applications. For more information on your potential research needs or goals contact Dr. McClintock by phone (703-927-9090) or EMAIL at DNADiagnosticsInc@gmail.com.
Virginia Department of Forensic Science, Roanoke, VA. This study is investigating the use of an over-the-counter product that is used in sexual assault casework within the Commonwealth of Virginia. The active ingredient in this product is used to treat redness of the eyes due to minor irritation by causing vasoconstriction of the conjunctival blood vessels. However, when orally ingested, the active ingredient activates the central alpha-2-adrenergic receptor which can lead to central nervous system depression and adverse cardiovascular abnormalities. Recently, this compound has been detected in the urine of sexual assault victims and it is unclear whether this drug was self-administered or involuntarily ingested. The goal of this research is to determine how long the active ingredient is detectable in urine after therapeutic ocular dosing. Participants of this study (Liberty University Undergraduate Students), administered the product to the conjunctival sac of each eye, 30 seconds apart, at times 0, 4, 8, and 12 hours. This method and the use of student volunteers was pre-approved by Liberty University’s Institutional Review Board and the Virginia Department of Forensic Science. This compound was isolated from urine by solid phase extraction and detected using a gas chromatograph mass spectrometer (GC/MS). Qualitative analysis was performed using GCMS by selective ion monitoring (SIM), which targets specific ion to ion ratios of the active ingredient in order to accurately identify the presence of the entire molecule. It is speculated that this research will provide new information for future law enforcement officers investigating sexual assault cases to determine if the product may have been involved in a drug facilitated sexual assault.
Creation Science Ministries of Oregon, Portland, OR. Between 1880-1947, several hundred mummified bodies were excavated from burial sites in the Paracas peninsula of Peru. However, since the discovery of these bodies many anthropologists and archaeologists have been unable to agree on the genetic origins suggesting that the "Cone Head" mummies’ cranial deformation points to another human species, an unknown hominid species, or an alien. The goal of this project is to investigate the hair structure and the genetic profiles from hair and tissue samples derived from the Paracas skulls. Hair and tissue samples were subjected to comparative hair analysis, raman spectrography, and DNA typing using short tandem repeat (STR) analysis. Comparative microscopic hair analysis and raman spectrography of samples from the Cone Head mummies and humans of varying ethnicities revealed very similar hair structure and characteristics associated with human hair. Following DNA extraction, the DNA was amplified using polymerase chain reaction (PCR), and the amplified STR alleles separated (Genetic Analyzer 3500 and GlobalFilier Software) using capillary electrophoresis. The results demonstrated allelic profiles or responses similar and consistent to those DNA profiles observed in humans. Moreover, no foreign DNA or unusual patterns/profiles were observed in any of the samples tested. Although these results need to be replicated and more analysis undertaken the data demonstrate that the Cone Head mummies (or the Paracas civilization) are humans and not an unknown hominid species or alien.
Virginia Department of Conservation and Recreation at Sailor's Creek Battlefield State Park, Rice, VA. Forensic DNA analysis has gained much notoriety over the past decade as a tool in human identification. DNA analysis has recently been used to investigate samples of historical significance. During the last battle of the American Civil War (Sailor’s Creek Battlefield, Rice, VA), the Hillsman House served as a medical and surgical center for wounded soldiers treating approximately 358 Union and 161 Confederate soldiers over a twenty-four hour period. The prominent “bloodstains” on the floor under the single surgical table and two post-surgical beds provides evidence of the vast number of soldiers treated. These presumed bloodstains, which also found their way through the cracks in the wood floors onto the supporting floor joists, were collected and subjected to various classic and state-of-the-art techniques to demonstrate the presence of blood. To examine the genomic profiles, DNA was isolated from these samples, quantitated, amplified using the GlobalFiler™ PCR Amplification Kit, and subjected to capillary electrophoresis. The generation of partial and/or complete DNA profiles confirmed the presence of human DNA, as well as the ability of DNA profiling to reveal a part history from a battle fought over 150 years ago.
Wisconsin Stem Cell Research Institute, Inc., Madison, WI. Thirty cell pellets containing human embryonic stem cells were analyzed at 16 STR (Short Tandem Repeat) loci using the PowerPlex 16 BIO system (Promega Co., Madison, WI). The resulting DNA profiles were analyzed to determine the cell line’s genotype as well as its uniqueness and frequency in a given population.
Case Western Reserve University, Cleveland, OH. A two-year study was conducted on pairs of siblings who were concordant and discordant for diabetes and nephropathy. As part of the quality control efforts for this project, STR (Short Tandem Repeat) profiles were obtained from approximately 2600 individuals and compared to profiles from buffy coat DNA derived from an EDTA tube and to DNA extracted from immortalized B cell lines. Such comparisons allowed the research team to determine if potential errors occurred (i.e., the error rate) and the integrity of the study.
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